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n/a
deleted
4109 Posts |
 Posted - 01/14/2005 : 05:18:55
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Thank you Floop, I'll see the reaction with the White album.
RUSHMORE? I think I've never heard of that one, but I'll search.
Purify the colors, purify my mind,
and spread the ashes of the colors
over this heart of mine!
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Edited by - n/a on 01/14/2005 05:21:57 |
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ramona
"FB Quote Mistress"
USA
3988 Posts |
Posted - 01/14/2005 : 07:47:44
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Floop, why do I love you so? 
_____________________________________________________________________
You change all the lead sleepin' in my head to gold,
as the day grows dim, I hear you sing a golden hymn,
the song I've been trying to say.
Purify the colors, purify my mind,
and spread the ashes of the colors
over this heart of mine!
* * * *
http://prettycrabby.com |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/14/2005 : 11:40:06
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ramona - i don't think i can help you with that one. was it the quesadillas?
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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ramona
"FB Quote Mistress"
USA
3988 Posts |
Posted - 01/14/2005 : 11:43:35
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I think it was. I dream of you covered in cheese. Mmm. Cheese.
_____________________________________________________________________
Hold your mistake up,
Before they turn the summer into dust
If the children don't grow up -
Our bodies get bigger, but our hearts get torn up...
With my lightning bolts a glowin'
I can see where I am going.
Better look out below!
* * * * * * * * * * *
http://prettycrabby.com |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/14/2005 : 11:52:08
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make a quesadilla? this site is very helpful, for starters:
groups.yahoo.com/group/quesadillamaster/
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
Posted - 01/14/2005 : 11:57:47
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Hehe. No I mean how do you always make me laugh? Well that wasn't what I meant but feel free to answer that and my original query which was actually, "How do you make women love you?"
http://www.thefutureheads.co.uk/ |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/14/2005 : 12:09:33
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it's a gift from god.
no, it's not the case in real life these days so i can't help you with that one
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/14/2005 : 12:27:08
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thanks for the vote of confidence.
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
Posted - 01/14/2005 : 12:30:45
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I am honestly shocked that you feel you need it. But anyway I am glad to give it when it's the truth.
http://www.thefutureheads.co.uk/ |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
Posted - 01/29/2005 : 15:28:59
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Hey floop. If you type homers pet monkey into google image search, you get a picture titled floop. Go take a look. Why is that?
Love, love, my season |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/29/2005 : 17:38:58
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liar
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
Posted - 01/30/2005 : 04:07:23
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Seriously floop, how odd is that?
Love, love, my season |
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The King Of Karaoke
> Teenager of the Year <
USA
3759 Posts |
Posted - 01/30/2005 : 09:14:38
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Dear Floop, how are your skills of traversing the rough terrain of the great north western U.S. (with a fifty pound back pack, in inclimate weather, while wearing night vision goggles, and listening to a 37 year old man obsess about the legendary, but elusive Sasquatch)?
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Edited by - The King Of Karaoke on 01/30/2005 09:36:18 |
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kathryn
~ Selkie Bride ~
Belgium
15320 Posts |
Posted - 01/30/2005 : 09:26:48
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Dear Floop
Where have all the flowers gone?
 I still believe in the excellent joy of the Frank |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/30/2005 : 10:49:05
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quote:
Originally posted by The King Of Karaoke
Dear Floop, how are your skills of traversing the rough terrain of the great north western U.S. (with a fifty pound back pack, in inclimate weather, while wearing night vision goggles, and listening to a 37 year old man obsess about the legendary, but elusive Sasquatch)?
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i went on a pretty serious backpacking trip with my dad when i was a young lad. i think i could manage.
what are we bringing for food?
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/30/2005 : 10:49:36
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quote:
Originally posted by kathryn
Dear Floop
Where have all the flowers gone?
to California
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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VoVat
>> Denizen of the Citizens Band <<
USA
9168 Posts |
Posted - 01/30/2005 : 11:26:28
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Dear Floop,
Why do birds suddenly appear every time you are near?
"Reunion? Shit union!" |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/30/2005 : 11:28:45
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those are vultures
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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Daisy Girl
~ Abstract Brain ~
Belize
5305 Posts |
Posted - 01/30/2005 : 11:32:18
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| Floop, what are you and HPM going to do when you have so many kitty heads they wont' fit on one row? |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
Posted - 01/30/2005 : 11:42:53
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We already have the maximum.
Love, love, my season |
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n/a
deleted
4109 Posts |
Posted - 01/30/2005 : 11:55:01
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Dear Floop, have you ever had a seitan meal?
We have the answer to all your fears
It's short, it's simple, it's crystal dear
It's round about, it's somewhere here
Lost amongst our winnings |
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VoVat
>> Denizen of the Citizens Band <<
USA
9168 Posts |
Posted - 01/30/2005 : 11:56:48
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You mean like the souls of gluttons?
"Reunion? Shit union!" |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/30/2005 : 12:27:54
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quote:
Originally posted by rita
Dear Floop, have you ever had a seitan meal?
We have the answer to all your fears
It's short, it's simple, it's crystal dear
It's round about, it's somewhere here
Lost amongst our winnings
you know i only eat Satan meals
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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starmekitten
-= Forum Pistolera =-
United Kingdom
6370 Posts |
Posted - 01/30/2005 : 12:59:06
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Floop, other than PMA can you tell me an axample of another agent that induces differentiation and it's use?
you
me
we used to be on fire |
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Homers_pet_monkey
= Official forum monkey =
United Kingdom
17125 Posts |
Posted - 01/30/2005 : 13:05:41
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Yeah floop, I bet you can't.
Love, love, my season |
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starmekitten
-= Forum Pistolera =-
United Kingdom
6370 Posts |
Posted - 01/30/2005 : 21:42:07
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ok, how about if I've done two colourmetric assays on cells treated with varying concentration foetal calf serum (FCS) and the crystal violet assay run through a spectrophotometer shows that the more FCS added the higher the absorbance at 540nm therefore the more cells there and hence higher proliferation in increased growth factor. BUT the 3-(4,5-dimethylthiazol-2-yl)-2,diphenyl tetrazolion bromide (MTT) assay shows a decreased absorbance inversely proportional to FCS concentration SO does this mean that the more FCS added the higher the rate of proliferation there will be but growth factors have a negative effect on cell viability; or does it mean my results are fucked?
floop?
you
me
we used to be on fire
[edit] just in case it wasn't 100% clear before |
Edited by - starmekitten on 01/30/2005 22:01:42 |
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Superabounder
* Dog in the Sand *
USA
1041 Posts |
Posted - 01/30/2005 : 21:52:21
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thank god I dropped out of pre-med. I would have got my ass kicked.
I know that a lot of what I say has been lifted off of men's room walls |
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starmekitten
-= Forum Pistolera =-
United Kingdom
6370 Posts |
Posted - 01/30/2005 : 22:04:45
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(fuck it's tetrazolium not tetrazolion and this was read at 570nm if that helps...)
So superbounder.. any clues there? no one is awake for me to bully here yet, sorry I mean compare results with of course...
you
me
we used to be on fire |
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floop
= Wannabe Volunteer =
Mexico
15297 Posts |
Posted - 01/30/2005 : 22:13:34
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quote:
Originally posted by starmekitten
ok, how about if I've done two colourmetric assays on cells treated with varying concentration foetal calf serum (FCS) and the crystal violet assay run through a spectrophotometer shows that the more FCS added the higher the absorbance at 540nm therefore the more cells there and hence higher proliferation in increased growth factor. BUT the 3-(4,5-dimethylthiazol-2-yl)-2,diphenyl tetrazolion bromide (MTT) assay shows a decreased absorbance inversely proportional to FCS concentration SO does this mean that the more FCS added the higher the rate of proliferation there will be but growth factors have a negative effect on cell viability; or does it mean my results are fucked?
floop?
you
me
we used to be on fire
[edit] just in case it wasn't 100% clear before
i think it's the former
ist es möglich für ein quesadilla skrotum zu lecken? beim sprechen der quesadillas von LBF, ja. ja in der tatheheheheheheehehee! |
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starmekitten
-= Forum Pistolera =-
United Kingdom
6370 Posts |
Posted - 01/30/2005 : 22:27:09
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You see I thought so, the way I figured it was that whilst FCS causes the cells to divide at a higher rate it's probably pushing them through the resriction point at the end of G1 phase before they've had time to grow they're shoved into S phase and then again rapidly through G2 because of the increased signalling from the growth factors to divide and they aren't getting much of a chance to synthesize DNA (S phase) or express proteins (G2) such as those needed to break down the MTT (mitochondrial dehydrogenases) to the blue formazan that is recorded at 570nm. Looking more closely around 5 and 1% FCS the drop off of absorbance is less, so it is likely there is an optimum FCS level for the cells which I would have thought was higher than this because of the 10% standard FCS added to a lot of media...
But if you says so floopster I believes you! There's only 20% of this module riding on this one...
you
me
we used to be on fire |
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Perk
- FB Fan -
USA
210 Posts |
Posted - 01/30/2005 : 22:51:44
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starmekitten
How do you feel about sea monkeys ?
Have they been bio engineered for the comsumer,or
just the same million year old creature. :p
Don't sweat the petty things
and don't pet the sweaty things |
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starmekitten
-= Forum Pistolera =-
United Kingdom
6370 Posts |
Posted - 01/30/2005 : 23:38:37
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I'm of the opinion they're a bunch of ugly gang banging necrophiliacs myself there perk...
you
me
we used to be on fire |
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Ask floop a question!
